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live cell imaging
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Live cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. Live cell imaging was pioneered in first decade of the 20th century. One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg. Since then, several microscopy methods have been developed which allow researchers to study living cells in greater detail with less effort. A newer type of imaging utilizing quantum dots have been used as they are shown to be more stable.
== Phase contrast microscopy ==
(詳細はstained to be visible in a traditional light microscope. Unfortunately, the process of staining cells generally kill the cells. With the invention of the phase contrast microscopy it became possible to observe unstained living cells in detail. After its introduction in the 1940s, live cell imaging rapidly became popular using phase contrast microscopy. The phase contrast microscope was popularized through a series of time-lapse movies (Video 1), recorded using a photographic film camera. Its inventor, Frits Zernike, was awarded the Nobel Prize in 1953.〔(【引用サイトリンク】 publisher=Nobel Media AB )〕 Other later phase contrast techniques used to observe unstained cells are Hoffman modulation and differential interference contrast microscopy.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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